Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM) - Rat
Catalog no : RS01P0278
Application : SDS-PAGE; WB; ELISA; IP.
Organism : Rat
Purity : > 95%
Host : E.coli
Expression System : Prokaryotic expression
Mol. Mass : 57.6kDa
Chemical Formula : n/a
Rsidues : Trp28~Lys527 (Accession # O35112) with N-terminal His-Tag
Assembled by : Uscn Life Science Inc.Wuhan
Formulation : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
Usage : Reconstitute in sterile PBS, pH7.2-pH7.4.
Sequences : The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-WYT VNSAYGDTIV MPCRLDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL SLSENYTLSI NNAKISDEKR FVCMLVTEDN VFEAPTLVKV FKQPSKPEIV NRAAFLETEQ LKKLGDCISR DSYPDGNITW YRNGKVLQPV DGEVSILFKK EIDPGTQLYT MTSSLEYKTT KSDIQMPFTC SVTYYGPSGQ KTIYSEQAIF DIYYPTEQVT IQVLPPKNAI KEGDNITLQC LGNGNPPPEE FMFYLPGQAE GIRSSNTYTL TDVRRNATGD YKCSLIDQRN MAASTTITVH YLDLSLNPSG EVTKQIGDTL PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV SAISIPEHDE ADDISDENRE KVNDQAK
Storage and Stability :
- Storage : Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
- Stability Test : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the Marker (complimentary) :
- Effective Size Range : 10kDa to 70kDa.
- Protein bands : 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
- Double intensity bands : The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
- Ready-to-use : No need to heat, dilute or add reducing agents before use.