Vitamin B12 (VB12, Cyanocobalamin) BioAssay™ ELISA Kit (Food)

Rs. 76,000.00
Make: US Biological Life Sciences
Vitamin B12 as a trace element belongs to the biologically important chelate formers. The basic unit consists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide, and a dimethyl benzimidazole group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the absence of intrinsic factor. Pernicious anemia can be treated by a high dosage of vitamin B12.
The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation. With the present test kit, it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sample pretreatment) compared with a conventional microbiological assay (24 to 48 hours).
Intended Use:
  • For the quantitative determination of Vitamin B12 in food.
  • Sensitivity: 0.3ng/ml
  • Recovery (spiked samples): ~98%
  • Incubation Time: 80 min

Kit Components:

V2126-01A: Microtiter Plate, 1x96 wells each, coated with anti-vitamin B12. V2126-01B: Standard 0ng/ml, 1x500ul, ready to use. V2126-01C: Standard 0.4ng/ml, 1x500ul, ready to use. V2126-01D: Standard 1ng/ml, 1x500ul, ready to use. V2126-01E: Standard 4ng/ml, 1x500ul, ready to use. V2126-01F: Standard 10ng/ml, 1x500ul, ready to use. V2126-01G: Standard 40ng/ml, 1x500ul, ready to use. V2126-01H: Vitamin B12 (HRP), 1x6ml, ready-to-use. V2126-01J: Substrate Solution (TMB), 1x15ml, ready to use. V2126-01K: Stop Solution (0.5M H2SO4), 1x15ml, ready to use. V2126-01L: Sample Diluent, 2x60ml, ready to use. V2126-01M: Washing Solution, 10x, 1x30ml

Storage and Stability:

  • Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
  • Sensitivity: 0.3ng/ml
  • Recovery (spiked samples): ~98%
  • Incubation Time: 80 min
  • Intra-assay Precision: 3%

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